Specs:

 SDS

Molecular Weight: 532.50; single compound

CAS: 756526-03-1

Purity: > 97%

Spacers: dPEG® Spacer is 19 atoms and 21.568 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease. Jiang Xia, Elin Bergseng, Burkhard Fleckenstein, Matthew Siegel, Chu-Young Kim, Chaitan Khosla and Ludvig M. Sollid. Bioorganic & Medicinal Chemistry. 15 (2007) pp 6565–6573. July 25, 2007. DOI: 10.1016/j.bmc.2007.07.001.

Design of a Potent D-Peptide HIV-1 Entry Inhibitor with a Strong Barrier to Resistance. Brett D. Welch, J. Nicholas Francis, Joseph S. Redman, Suparna Paul, Matthew T. Weinstock, Jacqueline D. Reeves, Yolanda S. Lie, Frank G. Whitby, Debra M. Eckert, Christopher P. Hill, Michael J. Root, and Michael S. Kay. Journal of Virology. 2010, 84 (21) pp 11235–11244. November 2010. DOI: 10.1128/JVI.01339-10.

Ligand-regulated oligomerization of b2-adrenoceptors in a model lipid bilayer. Juan Jose´ Fung, Xavier Deupi, Leonardo Pardo, Xiao Jie Yao, Gisselle A Velez-Ruiz, Brian T DeVree, Roger K Sunahara and Brian K Kobilka. The EMBO Journal, 2009, 28, pp 3315–3328. September 17, 2009. DOI: 10.1038/emboj.2009.267.

Monitoring the switching of single BSA-ATTO 488 molecules covalently end-attached to a pH responsive PAA brush. Namik Akkilic, Robert Molenaar, Mireille M.A.E. Claessens, Christian Blum, and Wiebe M. de Vos. Langmuir. 2016, pp 1-10. August 15, 2016. DOI: 10.1021/acs.langmuir.6b01064.

G-Quadruplex Induction by the Hairpin Pyrrole–Imidazole Polyamide Dimer. Shunsuke Obata, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, and Hiroshi Sugiyama. Biochemistry. 2018, 57, pp 498-502. December 13, 2017. DOI: 10.1021/acs.biochem.7b01059.

Surface Modification on Acoustic Wave Biosensors for Enhanced Specificity. Onursal Onen, Asad A. Ahmad, Rasim Guldiken and Nathan D. Gallant. Sensors. 2012, 12 pp 12317-12328. September 10, 2012. DOI: 10.3390/s120912317.

Surface Functionalization and Analysis Thereof for an Ovarian Cancer Diagnostic Biosensor. Asad Ali Ahmad. USF Scholor Commons. 2011, 1 (1) pp 297 416. June 21, 2011. DOI: scholarcommons.usf.edu/etd/2977.

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics. Andrzej S. Pitek, Slater A. Jameson, Frank A. Veliz, Sourabh Shukla, and Nicole F. Steinmetz. Biomaterials. 2016, 89 pp 89-97, February 23, 2016. DOI: 10.1016/j.biomaterials.2016.02.032.

Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli. Sven Reimann, Gereon Poschmann, Kerstin Kanonenbera, Kai Stuhler, Sander H.J. Smits, and Lutz Schmitt. Biochemical Journal. 2016, June 8, 2016. DOI: 10.1042/BCJ20160154.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

Toward the Development of GE11-Based Radioligands for Imaging of Epidermal Growth Factor Receptor-Positive Tumors. Benedikt Judmann, Diana Braun, Ralf Schirrmacher, Björn Wängler, Gert Fricker, and Carmen Wängler. ACS Omega 2022, 7, 31, 27690–27702. 2022. https://doi.org/10.1021/acsomega.2c03407

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

Additional Search Phrases:

Bis-dPEG5-NHS ester, n-hydroxysuccinimide, 756526031, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, crosslinker, cross linker, crosslinking reagent, amine reactive, homobifunctional, NHS, N-hydroxysuccinimide, NHS ester, N-hydroxysuccinimide ester, active ester, Bis-PEG5-NHS ester, NHS-PEG5-NHS ester, Bis(succinimidyl) penta(ethylene glycol), BS(PEG)5, Bis-NHS ester- PEG



 SDS    Datasheet

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Bis-dPEG®₅-NHS ester

$158.00 – $457.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email [email protected] for Bulk Pricing and Custom Syntheses
Product#: 10224 Categories: ,

TFP_Esters_Art2

Bis-dPEG®5-NHS ester, product number 10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Bis-dPEG®5-NHS ester, product number 10224, has been published in the scientific literature. A list of relevant publications for this product appears at the end of this product description.

Homobifunctional Reagents

Homobifunctional reagents are chemical compounds that contain two identical reactive groups. Symmetrical homobifunctional reagents were among the first crosslinkers developed for conjugation of biomolecules. These were typically hydrophobic compounds, and their use – particularly in one-step reactions – often was accompanied with problems such as inactivation and precipitation of the conjugated biomolecules. Greg Hermanson’s comprehensive reference work Bioconjugate Techniques has an entire chapter (Chapter 5) devoted to homobifunctional reagents. Quanta BioDesign, Ltd. recommends and sells this authoritative reference.

Quanta BioDesign’s products are amphiphilic. Therefore, they impart water solubility to molecules to which they are conjugated. Consequently, when used in crosslinking reactions, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation. For a thorough explanatory discussion of dPEG® products, please visit our “What is dPEG®?” page. For answers to our frequently asked questions, please click this link to visit our page “Frequently Asked Questions (about dPEG® products).”

Using Bis-dPEG®5-NHS ester

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.0. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases.

Thus, we strongly discourage storing Bis-dPEG®5-NHS ester, product number 10224, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Commercial Scale Production of Bis-dPEG®5-NHS ester Is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Buy It Now!

Hydrophobic crosslinkers trigger aggregation and precipitation of biomolecules and contribute to problems with non-specific binding. Polymeric PEG crosslinkers eliminate the issues caused by hydrophobic crosslinkers, but they create new, difficult challenges in analyzing and characterizing the crosslinked conjugates.

You can solve all of those problems right now by clicking on the “Add to Cart” button and purchasing Bis-dPEG®5-NHS ester. The monodispersed PEG crosslinker provides water solubility and larger hydrodynamic volume to the conjugate molecules. However, because it is a discrete compound, not a polymer, the analytical challenges caused by dispersed polymers disappear.

So stop using inferior crosslinkers! Click the “Add to Cart” button now to order Bis-dPEG®5-NHS ester.

Description

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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